42:, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a
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of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the
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Determining the viable cell count is important for calculating dilutions required for the
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to determine the number of living cells in a culture. This is different from other
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A dilution of the cells to be counted is prepared and mixed with
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Method used to determine number of living cells in a cell culture
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because it makes a distinction between live and dead cells.
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80:"Invitrogen viable cell count method"
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28:cell counting techniques
104:"Plaque assay protocol"
22:is a method used in
50:Use in Cell Culture
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134:Cell culture
110:. Retrieved
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86:. Retrieved
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60:plaque assay
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24:cell culture
20:Viable count
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40:Trypan blue
66:References
56:passaging
128:Category
112:4 June
88:4 June
34:Method
107:(PDF)
83:(PDF)
114:2013
90:2013
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