Knowledge

Immunophenotyping

Source 📝

85:
Immunophenotyping is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity. This technique allows rapid and easy phenotyping of each cell in a heterogeneous sample according to the presence or absence
33:
is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue),
70:, a laser-based instrument capable of analyzing thousands of cells per second. The whole procedure can be performed on cells from the 103:
Young, Yoon Kow; Bolt, Alicia M.; Ahn, Ryuhjin; Mann, Koren K. (2016). "Analyzing the Tumor Microenvironment by Flow Cytometry".
124: 159: 17: 108: 169: 185: 165: 8: 190: 138: 130: 120: 112: 51: 35: 116: 67: 62:
on their membrane. By choosing appropriate antibodies, the differentiation of
179: 134: 39: 142: 79: 66:
cells can be accurately determined. The labelled cells are processed in a
75: 43: 63: 55: 47: 59: 71: 177: 102: 160:British Society for Haematology guidelines 168:at the U.S. National Library of Medicine 38:, etc. An example is the detection of 14: 178: 27:Classifying cells of the immune system 111:. Vol. 1458. pp. 95–110. 24: 25: 202: 153: 50:. It involves the labelling of 96: 13: 1: 89: 109:Methods in Molecular Biology 82:in a matter of a few hours. 7: 117:10.1007/978-1-4939-3801-8_8 86:of a protein combination. 10: 207: 105:The Tumor Microenvironment 58:directed against surface 170:Medical Subject Headings 162:accessed July 31, 2006 166:Immunophenotyping 126:978-1-4939-3799-8 52:white blood cells 42:, such as in the 31:Immunophenotyping 16:(Redirected from 198: 147: 146: 100: 21: 206: 205: 201: 200: 199: 197: 196: 195: 176: 175: 156: 151: 150: 127: 101: 97: 92: 36:cell suspension 28: 23: 22: 18:Immunophenotype 15: 12: 11: 5: 204: 194: 193: 188: 186:Flow cytometry 174: 173: 163: 155: 154:External links 152: 149: 148: 125: 94: 93: 91: 88: 68:flow cytometer 26: 9: 6: 4: 3: 2: 203: 192: 189: 187: 184: 183: 181: 171: 167: 164: 161: 158: 157: 144: 140: 136: 132: 128: 122: 118: 114: 110: 106: 99: 95: 87: 83: 81: 77: 73: 69: 65: 61: 57: 53: 49: 45: 41: 40:tumor markers 37: 32: 19: 104: 98: 84: 80:spinal fluid 30: 29: 76:bone marrow 191:Hematology 180:Categories 90:References 56:antibodies 135:1940-6029 44:diagnosis 143:27581017 64:leukemic 60:proteins 48:leukemia 172:(MeSH) 141:  133:  123:  72:blood 54:with 139:PMID 131:ISSN 121:ISBN 113:doi 78:or 46:of 182:: 137:. 129:. 119:. 107:. 74:, 145:. 115:: 20:)

Index

Immunophenotype
cell suspension
tumor markers
diagnosis
leukemia
white blood cells
antibodies
proteins
leukemic
flow cytometer
blood
bone marrow
spinal fluid
Methods in Molecular Biology
doi
10.1007/978-1-4939-3801-8_8
ISBN
978-1-4939-3799-8
ISSN
1940-6029
PMID
27581017
British Society for Haematology guidelines
Immunophenotyping
Medical Subject Headings
Categories
Flow cytometry
Hematology

Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.