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In-ovo sexing

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233:, Netherlands. This company was founded in 2013 by students Wouter Bruins (biology) and Wil Stutterheim (biomedical sciences) and, just like Seleggt, uses a small amount of liquid from the hatching egg and determines whether it is a male or female hatching egg by means of biomarker detection on the 9th day. By January 2020, In Ovo was capable of sexing 1,500 eggs an hour (0.42 per second), but the Dutch poultry sector required 40,000 eggs an hour, so further innovation was necessary. In Ovo received millions of euros in research investments, mostly from German chemicals company Evonik, Singaporese venture capital company Visvires New Protein, and 132:
More animal friendly because the day-old male chicks no longer need to be culled, and more sustainable because less energy is used because only the female eggs need to be further incubated after sexing. The male eggs are sorted out and can be used for different purposes such as an alternative high-value protein source. When there are no longer males that hatch, other innovations are also available to egg producers, such as on-farm hatching and in-ovo vaccination.
123:, both for human consumption; after the sexing, these female chicks are transported to the rearing farms where they are housed before they go to a laying hen farm or broiler farm. On the other hand, the male chicks are deemed to have much less economic value, as they cannot lay eggs and are usually less suited for meat production; most male chicks are therefore culled on the day they hatch after they have been sexed as male. 187:
consumption eggs that are laid by the hens that have been sexed with the Seleggt method are to be found on the shelves of the German supermarket REWE, in the Berlin region. The eggs that have been sexed with the Seleggt method are sold under the label "Respeggt". This label guarantees the promise "Free of chick culling". Since 2018, Respeggt eggs have been available in Germany, France, and the Netherlands.
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explored in 2017. When the pattern analysis in hyperspectral images methodology was researched. First, the eggs would be candled with halogen lamps. Then, a hyperspectral camera would collect the transmitted light and the eggs would be classified using a linear discriminant analysis. This methodology could perform in-ovo sexing from 11-day up to 14-day embryos with a 97% accuracy.
22: 222:' for the sex through a small hole in the eggshell on day 9 after fertilisation. Mixed with fluid from fertilised eggs, this marker changes blue for a male and white for a female, with a 98.5% accuracy rate. As of May 2019, Seleggt sexed one egg per second (3,600 an hour) and thus enabled 30,000 'no-kill' female chicks to hatch in Germany every week. The 196:
in-ovo sexed chick is estimated to cost around $ 3.80, compared to $ 0.95 for a conventional chick. Since a chick lays around 350 eggs over its life, this means an additional 0.8 cents per table egg sold. These eggs then sell for around 1-3 additional cents per table egg, the difference including the additional margins from each part of the supply chain.
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spectroscopy,  and endocrine method. The main drawbacks of invasive procedures is that they may affect the eggs’ hatchability and that the process implies a risk for bacterial infection. On the other hand, non-invasive technologies, have a contactless in-ovo sexing procedure (e.g. AI-powered imaging) are free of this previous risks.
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Since the launch of in-ovo sexing in 2018, multiple companies have had success in Europe, implementing different methodologies and technologies for in-ovo sexing. As of September 2023, it was estimated that 15 per cent of the layer flock in Europe used in-ovo sexing, from five different companies. An
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In-ovo gender determination has the potential to bring an end to the killing of billions of male chicks. It is estimated that yearly around 7 billion day-old male chicks are killed. Implementing in-ovo sexing into the poultry industry results in a more animal friendly and more sustainable production.
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to determine the sex of the egg. The hatching egg is examined with by light beam, with a hyperspectral measuring technology the sex is determined on the basis of the calculated light spectrum. This method works for hatching eggs from sex-linked breeds where male and female birds have different color
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Research has been conducted to achieve this goal. The first study on the matter, was published in 2013, for in-ovo sexing on day 9 of incubation. The researched procedure, later called bio-marker detection, used a hormonal test for the allantoic fluid of brown layers’ eggs. Further research was done
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In January 2021, Germany was the first country to successfully outlaw the practice. A few months later, France also banned the one-day-old male chick slaughter. In 2022, the third country, Italy, followed the same steps, prohibiting the practice by 2026. In 2024, the UK government's official Animal
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Genus Focus has different installations across Europe, including France, Germany, the Netherlands and soon Norway. Orbem has entered a strategic partnership with the Vencomatic Group (NL) for end-to-end automation of the process, which includes financing facilitated by the Vencomatic Group of up to
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Based on years of scientific research at the interface of AI, imaging technology and embryonic poultry development, Orbem was founded in 2019 as a spin-off from the Technical University of Munich. Orbem uses AI-powered MRI to conduct in-ovo sexing on day 11 - 12 of incubation, with a throughput of
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The in-ovo sexing technologies have evolved into two main categories: invasive and non-invasive. An invasive technology is any technology that creates damage to the egg through intrusive sampling and biochemical analysis for the in-ovo sexing process. Examples of these are biomarker detection, PCR,
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Hypereye is a Canadian spectroscopic technology that is being developed by the Egg Research Development Foundation (ERDF), initially funded by Poultry Industry Council in Ontario and later by Egg Farmers of Ontario (EFO). It aims to achieve a 99% accuracy rate and to process 30,000–50,000 eggs per
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Krautwald-Junghanns, ME; Cramer, K; Fischer, B; Förster, A; Galli, R; Kremer, F; Mapesa, EU; Meissner, S; Preisinger, R; Preusse, G; Schnabel, C; Steiner, G; Bartels, T (1 March 2018). "Current approaches to avoid the culling of day-old male chicks in the layer industry, with special reference to
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methodology was developed, it analyzes the extraembryonic blood to determine the sex of the embryo through its blood wavelength. The supervised egg classification by a PC with a 93% error rate was able to determine the sex of 380 eggs at 3.5 incubation day. A variation of this methodology was
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In 2019, a new methodology was developed, AI-powered imaging. By combining AI and MRI together to perform in-ovo sexing of 12-day-old eggs with 95% accuracy level. Since 2007 MRI was used as a valuable tool for studying egg development in a contactless manner. Yet it would not be until it was
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In 2018, Seleggt was the first company to succeed in commercial in-ovo sexing. Seleggt managed to sex the hatching eggs on day 9 of the incubation process with a hormone test. The method is based on the fundamental research of Prof. Dr. Einspanier at Leipzig University. From 8 November 2018,
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plumage, all males white-feathered and all females brown-feathered for example, and can take place from the 13th day of the hatching process. AAT's goal is to eventually be able to sex eggs at the 4th day. The German Agriculture Ministry has also invested in AAT's technological development.
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The German company Plantegg uses a PCR method, which uses DNA to determine whether the hatching egg is male or female. Like In Ovo and Seleggt, this method determines the sex on day 9 of the incubation process. This method is expected to be ready for use by the end of 2020.
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In the US, male culling has received substantial attention from animal activists, although in-ovo sexing is not currently available. However, the egg industry has indicated that they will strongly support the adoption of this technology once it is available.
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For a long time, it was held impossible to determine the sex of the hatching egg before or during the hatching process. The poultry sector has been working on this for years in order to be able to phase out chick culling in the interest of animal welfare.
275:(CNRS) on Projet Soo, which employs a mix of spectroscopy and the use of biosensors with the target of achieving 90% accuracy in ovo sexing at 9 days of incubation by the end of 2019. In 2017, French Agriculture Minister 300:
15 million Euros for equipment to be deployed at customer sites throughout Europe. This allows hatcheries to use the combined solution without any upfront investment just based on a performance fee per egg.
627: 801:"In-ovo sexing of 14-day-old chicken embryos by pattern analysis in hyperspectral images (VIS/NIR spectra): A non-destructive method for layer lines with gender-specific down feather color" 107:
of one-day-old male chicks, billions of male chicks that are killed as part of the production process each year. At the day the chicks hatch from their eggs the chicks are sexed. During
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Doran T. J., Morris K. R., Wise T. G., O'Neil T. E., Cooper C. A., Jenkins K. A., Tizard M. L. V. (2017) Sex selection in layer chickens. Animal Production Science 58, 476-480.
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by Prof. Dr. Einspanier following the same methodology. It is important to mention that hatchability is affected by this in-ovo sexing method, as reported by Dr. Einspeiner.
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In-ovo sexing technology has branched into two categories, invasive and non-invasive. The first invasive technology to be successfully commercially introduced for
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Galli, Roberta; Preusse, Grit; Uckermann, Ortrud; Bartels, Thomas; Krautwald-Junghanns, Maria-Elisabeth; Koch, Edmund; Steiner, Gerald (1 February 2017).
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up to 24,000 eggs per hour. The solution applies equally to brown and white eggs. The solution is contactless and has no effect on the hatchability rate.
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Reithmayer, C., & Mußhoff, O. (2019). Consumer preferences for alternatives to chick culling in Germany. Poultry science, 98(10), 4539–4548
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in November 2018. Meanwhile, the non-invasive in-ovo sexing technologies have been introduced to the market by the German start-up
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Ching, Congo Tak Shing; Wang, Chien-Kai; Li, Chin; Chiu, Hsuan-Ni; Hieu, Nguyen Van; Phan, Thien Luan (12 November 2021).
883:"Noninvasive monitoring of chick development in ovo using a 7T MRI system from day 12 of incubation through to hatching" 628:"Maharashtra Joins List of States Committing to Adopting In Ovo Sexing Technology to Prevent Killing of Male Chicks" 1278: 1071: 550: 524: 141:
Welfare Committee recommended that the UK ban chick culling, although their recommendation is not legally binding.
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Bain, Maureen M.; Fagan, Andrew J.; Mullin, James M.; McNaught, Iain; McLean, John; Condon, Barrie (July 2007).
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A number of state governments in India have mandated that in-ovo sexing be used once it becomes available.
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Tweel, L. R. G. van den; Pol, C. W. van der; Brand, H. van den; Neethirajan, S. R. (11 February 2022).
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the day-old chicks are divided into male and female groups. Female chicks can be raised to become
1507:"Vencomatic Group and Orbem announce a strategic partnership for in ovo sexing of poultry eggs" 1176:"Non-invasive in-ovo sexing of chicken eggs using Raman spectroscopy and hyperspectral imaging" 1549: 1147: 717: 8: 1403: 665: 648:
Weissmann, A.; Reitemeier, S.; Hahn, A.; Gottschalk, J.; Einspanier, A. (1 August 2013).
1047:"In-ovo sexing reaches 15% penetration in EU as tech to end male chick culling advances" 287:
announced an $ 844,000 investment in the research project to stimulate its development.
276: 1135: 1123: 922: 838: 781: 685: 499: 1231: 1139: 914: 830: 822: 773: 765: 677: 669: 650:"Sexing domestic chicken before hatch: A new method for in ovo gender identification" 284: 230: 58: 926: 842: 689: 1127: 904: 894: 812: 785: 757: 661: 268: 219: 1131: 1352: 1228:"Millions invested in Leiden method of determining sex of chicks before hatching" 69: 30: 1096: 761: 575: 1543: 1318: 826: 769: 673: 104: 98: 745: 918: 834: 777: 681: 260: 226:
has invested 5 million euros in the development of the Seleggt technology.
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granted Projet Soo 4.3 million euros to finance its egg sexing research.
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hour (8.3–13.9 eggs per second). In 2018, Canadian Agriculture Minister
1023: 899: 882: 57:"). There are various methods to determine a chick's sex in the 21-day 1429: 1404:"Germany, France push to end male chick 'shredding' in European Union" 909: 1253:"Pasgeboren kuikens niet meer vergast of in de shredder in Frankrijk" 1202:"Germany hopes new technology will stop the slaughter of male chicks" 62: 551:"Why the US egg industry is still killing 300 million chicks a year" 234: 81: 500:"Italy Outlaws Slaughter of Male Chicks, Saving Millions of Lives" 448:"Germany Becomes First Country to Ban Mass Culling of Male Chicks" 799:
Göhler, Doreen; Fischer, Björn; Meissner, Sven (1 January 2017).
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The Dutch–German company Seleggt measures a substance that is a '
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combined with AI that it could be used for large-scale purposes.
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Another German company, Agri Advanced Technologies (AAT), uses
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Gibbs, C. S. (2016). A guide to sexing chicks. Read Books Ltd.
50: 746:"In ovo sexing of chicken eggs by fluorescence spectroscopy" 743: 120: 73: 42: 72:
was bio-market detection through the Dutch–German company
1345:"Une technique pour éviter le broyage des poussins mâles" 54: 1173: 422:"In-ovo sexing opens door to on-farm hatching of layers" 21: 880: 602:"United Egg Producers Updated Statement on Male Chicks" 103:
The present-day ethical problem with egg production is
994:"Launched: Method to identify gender in hatching eggs" 224:
German Federal Ministry of Food and Agriculture (BMEL)
798: 1455: 151: 77: 1342: 126: 473:"France to outlaw culling of day-old male chicks" 322:"World's first no-kill eggs go on sale in Berlin" 1541: 1121: 229:Another Dutch company is In Ovo, a spin-off of 181: 1180:WIAS Annual Conference 2022: Collective Action 861:Foundation for Food & Agriculture Research 319: 273:French National Centre for Scientific Research 290: 965: 249: 199: 1375: 1182:. Wageningen University & Research: 47. 968:"Durchbruch: Gemeinsam KĂĽkentöten beenden!" 703: 701: 699: 315: 313: 1221: 1219: 1199: 1013: 908: 898: 816: 599: 255:Spectroscopy (AAT, Project Soo, Hypereye) 135: 1427: 1195: 1193: 1191: 1189: 991: 939: 707: 696: 548: 445: 310: 208: 20: 1481:"Orbem | Genus Focus for in-ovo sexing" 1456:"Orbem | Unleashing AI-powered imaging" 1371: 1369: 1216: 1072:"Opinion on chick culling alternatives" 525:"Opinion on chick culling alternatives" 16:Method to determine the sex of chickens 1542: 1316: 1225: 1044: 750:Analytical and Bioanalytical Chemistry 394:Fabian, Brockotter (7 February 2020). 393: 214:Bio-marker detection (Seleggt, In Ovo) 1504: 1395: 1343:Marie-NoĂ«lle Delaby (17 April 2019). 1186: 887:Journal of Magnetic Resonance Imaging 419: 396:"SELEGGT stops day-old-chick culling" 389: 387: 267:The French company Tronico, based in 1401: 1366: 666:10.1016/j.theriogenology.2013.04.014 497: 156: 1226:Epping, Marieke (29 October 2018). 1045:Watson, Elaine (13 December 2023). 549:Danovich, Tove K. (12 April 2021). 320:Josie Le Blond (22 December 2018). 13: 992:McDougal, Tony (9 November 2018). 625: 420:Yaman, Robert (16 November 2023). 384: 14: 1566: 1402:Wray, Meaghan (16 January 2020). 446:Nosowitz, Dan (9 December 2021). 152:Early technological breakthroughs 1376:Melanie Epp (19 December 2016). 1279:"ALDI schafft das KĂĽkentöten ab" 966:Seleggt GmbH (8 November 2018). 498:Rabb, Maxwell (15 August 2022). 127:Innovation in the poultry sector 1498: 1473: 1448: 1428:Willkomm, Eva (26 April 2021). 1421: 1336: 1310: 1296: 1271: 1245: 1167: 1115: 1089: 1064: 1038: 1007: 985: 959: 933: 874: 849: 792: 737: 641: 619: 593: 568: 542: 517: 491: 708:WeiĂźmann, Anne (3 June 2014). 465: 439: 413: 375: 357: 348: 339: 1: 303: 92: 80:and AAT, a subsidiary of the 182:First commercial application 7: 1528: 1259:(in Dutch). 29 January 2020 1132:10.21203/rs.3.rs-1047909/v1 600:uep_admin (25 March 2021). 10: 1571: 1378:"Hypereye: A game changer" 1200:David Reid (30 May 2019). 291:AI-powered imaging (Orbem) 119:fed to be slaughtered for 96: 87: 1382:Canadian Poultry Magazine 762:10.1007/s00216-016-0116-6 250:Non-invasive technologies 200:Sex determination methods 171:fluorescence spectroscopy 41:method carried out while 1097:"In-Ovo Sexing Overview" 576:"In-Ovo Sexing Overview" 364:spectroscopic methods". 271:, collaborates with the 1155:Cite journal requires 725:Cite journal requires 136:Male-chick culling ban 26: 1505:Orbem (31 May 2022). 209:Invasive Technologies 24: 606:United Egg Producers 1101:Innovate Animal Ag 972:Leipzig University 900:10.1002/jmri.20963 580:Innovate Animal Ag 479:. 23 February 2022 27: 1232:Leiden University 942:"Seleggt process" 818:10.3382/ps/pew282 285:Lawrence MacAulay 231:Leiden University 157:Academic research 115:-laying hens, or 59:incubation period 1562: 1522: 1521: 1519: 1517: 1502: 1496: 1495: 1493: 1491: 1477: 1471: 1470: 1468: 1466: 1452: 1446: 1445: 1443: 1441: 1425: 1419: 1418: 1416: 1414: 1399: 1393: 1392: 1390: 1388: 1373: 1364: 1363: 1361: 1359: 1340: 1334: 1333: 1331: 1329: 1314: 1308: 1307: 1300: 1294: 1293: 1291: 1289: 1275: 1269: 1268: 1266: 1264: 1249: 1243: 1242: 1240: 1238: 1223: 1214: 1213: 1211: 1209: 1197: 1184: 1183: 1171: 1165: 1164: 1158: 1153: 1151: 1143: 1119: 1113: 1112: 1110: 1108: 1093: 1087: 1086: 1084: 1082: 1068: 1062: 1061: 1059: 1057: 1042: 1036: 1035: 1033: 1031: 1026:on 25 March 2020 1022:. 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Retrieved 326:The Guardian 325: 298: 294: 281: 266: 261:spectroscopy 258: 244: 228: 217: 203: 194: 190: 189: 185: 176: 168: 164: 160: 147: 143: 139: 130: 109:chick sexing 102: 67: 39:chick sexing 34: 28: 18: 1535:Auto-sexing 1436:(in German) 1408:Global News 1349:Que Choisir 1544:Categories 1257:RTL Nieuws 1056:21 January 910:2262/29246 811:(1): 1–4. 431:21 January 304:References 93:Background 61:before it 45:are still 1283:Aldi Nord 1140:244095537 827:0032-5791 770:1618-2650 674:0093-691X 220:biomarker 1529:See also 1485:orbem.ai 1460:orbem.ai 1328:25 March 1319:"In ovo" 1304:"CHEGGY" 1107:18 March 1081:18 March 1030:25 March 1020:respeggt 999:25 March 951:25 March 927:30081472 919:17659540 843:24321843 835:27591278 778:27966169 690:21039185 682:23726296 626:sumant. 534:18 March 504:The Beet 405:25 March 235:Rabobank 117:broilers 82:EW Group 1490:29 July 1323:agri-at 786:3778005 633:29 June 611:29 June 585:29 June 560:29 June 88:History 74:Seleggt 63:hatches 1511:Medium 1413:22 May 1387:22 May 1358:22 May 1288:28 May 1263:22 May 1237:22 May 1208:22 May 1204:. CNBC 1138:  1076:GOV.UK 977:28 May 925:  917:  841:  833:  825:  784:  776:  768:  688:  680:  672:  529:GOV.UK 331:22 May 47:in ovo 43:chicks 1136:S2CID 923:S2CID 839:S2CID 782:S2CID 686:S2CID 78:Orbem 51:Latin 37:is a 25:Chick 1518:2022 1492:2024 1467:2022 1442:2022 1415:2020 1389:2020 1360:2020 1330:2020 1290:2020 1265:2020 1239:2020 1210:2020 1161:help 1109:2024 1083:2024 1058:2024 1032:2020 1001:2020 979:2020 953:2020 915:PMID 868:2022 831:PMID 823:ISSN 774:PMID 766:ISSN 731:help 678:PMID 670:ISSN 635:2023 613:2023 587:2023 562:2023 536:2024 511:2022 485:2022 459:2022 433:2024 407:2020 333:2020 121:meat 1128:doi 905:hdl 895:doi 813:doi 758:doi 754:409 662:doi 555:Vox 113:egg 55:egg 29:In 1546:: 1509:. 1483:. 1458:. 1432:. 1406:. 1380:. 1368:^ 1347:. 1321:. 1281:. 1255:. 1230:. 1218:^ 1188:^ 1178:. 1152:: 1150:}} 1146:{{ 1134:. 1126:. 1099:. 1074:. 1049:. 1018:. 970:. 944:. 921:. 913:. 903:. 891:26 889:. 885:. 859:. 837:. 829:. 821:. 809:96 807:. 803:. 780:. 772:. 764:. 752:. 748:. 722:: 720:}} 716:{{ 698:^ 684:. 676:. 668:. 658:80 656:. 652:. 604:. 578:. 553:. 527:. 502:. 475:. 450:. 424:. 398:. 386:^ 370:97 368:. 324:. 312:^ 84:. 65:. 33:, 1520:. 1494:. 1469:. 1444:. 1417:. 1391:. 1362:. 1332:. 1306:. 1292:. 1267:. 1241:. 1212:. 1163:) 1159:( 1142:. 1130:: 1111:. 1085:. 1060:. 1034:. 1003:. 981:. 955:. 929:. 907:: 897:: 870:. 845:. 815:: 788:. 760:: 733:) 729:( 712:. 692:. 664:: 637:. 615:. 589:. 564:. 538:. 513:. 487:. 461:. 435:. 409:. 335:. 49:(

Index


poultry farming
chick sexing
chicks
in ovo
Latin
egg
incubation period
hatches
poultry farming
Seleggt
Orbem
EW Group
Chick culling
chick culling
chick sexing
egg
broilers
meat
fluorescence spectroscopy
biomarker
German Federal Ministry of Food and Agriculture (BMEL)
Leiden University
Rabobank
spectroscopy
La Roche-sur-Yon
French National Centre for Scientific Research
Stéphane Le Foll
Lawrence MacAulay

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