202:
samples, so her and colleague Piotr
Chomczynski developed a new method to extract the nucleic acid. Since the publication of the technique in 1987 it has become ubiquitous in biological research, earning the paper the accolade of being one of the most cited in history.
221:
Sacchi has previously criticised the culture of science in her home nation of Italy, describing the system as “fairly corrupt” and ignorant of the achievements of female scientists. She believes that this has led to
436:
Chomczynski, Piotr; Sacchi, Nicoletta (August 2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on".
206:
Sacchi went back to the
University of Milan in 1991 and was promoted to professor. Unsatisfied with research in Italy she spent time back in the US as a visiting professor at the
126:
71:
401:
Chomczynski, Piotr; Sacchi, Nicoletta (1987-04-01). "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction".
490:
485:
377:
500:
270:
207:
247:
122:
102:
505:
194:
She moved to the US in 1982 and switched to cancer research, focussing on the analysis of the genetics of patient samples at
177:
214:. In 2003 she joined the Roswell Park Comprehensive Cancer Center where she continues to study the role of genetics and the
235:
495:
520:
338:
188:
515:
166:
165:. She remained within the fields of genetics for her postdoctoral training at the same University and
195:
211:
184:
510:
234:
In 2001 Sacchi was proclaimed a “Highly Cited
Researcher in Biology and Biochemistry” by the
525:
378:"Oral History | Life in Science | Women in Science | Nicoletta Sacchi on Scientific Career"
223:
8:
154:
61:
243:
461:
453:
418:
414:
346:
445:
410:
137:. As a consequence of this groundbreaking discovery she is now considered the most
306:
180:
130:
479:
457:
422:
350:
198:. This required a high yield technique to extract RNA from scarce paediatric
465:
239:
88:
42:
449:
215:
169:
199:
173:
158:
118:
92:
134:
162:
150:
138:
127:
acid guanidinium thiocyanate-phenol-chloroform extraction
72:
Acid guanidinium thiocyanate-phenol-chloroform extraction
117:(born August 20, 1949) is an Italian professor of
435:
400:
477:
339:"World class: Nicoletta Sacchi view from Italy"
336:
271:"Nicoletta Sacchi, la sua vita e i suoi studi"
491:Expatriate academics in the United States
208:Sydney Kimmel Comprehensive Cancer Center
486:Italian expatriates in the United States
311:Roswell Park Comprehensive Cancer Center
123:Roswell Park Comprehensive Cancer Center
103:Roswell Park Comprehensive Cancer Center
478:
172:. Sacchi worked on the generation of
372:
370:
368:
366:
332:
330:
328:
326:
301:
299:
297:
295:
293:
291:
265:
263:
236:Institute for Scientific Information
275:Video: ultime notizie - Corriere TV
13:
501:Italian expatriates in Switzerland
125:. She is the co-discoverer of the
14:
537:
363:
323:
288:
260:
153:and studied for her PhD at the
506:Italian expatriates in Germany
429:
394:
189:Basel Institute for Immunology
141:woman scientist in the world.
1:
337:Guardian Staff (2003-11-18).
253:
133:from biological samples with
415:10.1016/0003-2697(87)90021-2
229:
7:
157:in 1972, investigating the
10:
542:
496:University of Milan alumni
196:National Cancer Institute
144:
108:
98:
84:
77:
67:
57:
49:
28:
21:
521:Italian women scientists
246:awarded Sacchi with the
226:of trained researchers.
212:Johns Hopkins University
403:Analytical Biochemistry
218:on cancer development.
242:. In 2007 the Italian
450:10.1038/nprot.2006.83
174:antibody repertoires
516:Italian oncologists
155:University of Milan
149:Sacchi was born in
62:University of Milan
307:"Nicoletta Sacchi"
248:Commander of Merit
167:Erasmus University
39:September 20, 1949
16:Italian oncologist
112:
111:
79:Scientific career
533:
470:
469:
438:Nature Protocols
433:
427:
426:
398:
392:
391:
389:
388:
382:library.cshl.edu
374:
361:
360:
358:
357:
334:
321:
320:
318:
317:
303:
286:
285:
283:
282:
267:
115:Nicoletta Sacchi
38:
36:
23:Nicoletta Sacchi
19:
18:
541:
540:
536:
535:
534:
532:
531:
530:
476:
475:
474:
473:
434:
430:
399:
395:
386:
384:
376:
375:
364:
355:
353:
335:
324:
315:
313:
305:
304:
289:
280:
278:
269:
268:
261:
256:
232:
147:
58:Alma mater
45:
40:
34:
32:
24:
17:
12:
11:
5:
539:
529:
528:
523:
518:
513:
508:
503:
498:
493:
488:
472:
471:
444:(2): 581–585.
428:
409:(1): 156–159.
393:
362:
322:
287:
258:
257:
255:
252:
231:
228:
185:Georges Köhler
183:in the lab of
181:DNA polymerase
146:
143:
110:
109:
106:
105:
100:
96:
95:
86:
82:
81:
75:
74:
69:
68:Known for
65:
64:
59:
55:
54:
51:
47:
46:
41:
30:
26:
25:
22:
15:
9:
6:
4:
3:
2:
538:
527:
524:
522:
519:
517:
514:
512:
511:Living people
509:
507:
504:
502:
499:
497:
494:
492:
489:
487:
484:
483:
481:
467:
463:
459:
455:
451:
447:
443:
439:
432:
424:
420:
416:
412:
408:
404:
397:
383:
379:
373:
371:
369:
367:
352:
348:
344:
340:
333:
331:
329:
327:
312:
308:
302:
300:
298:
296:
294:
292:
276:
272:
266:
264:
259:
251:
249:
245:
241:
237:
227:
225:
219:
217:
213:
209:
204:
201:
197:
192:
190:
186:
182:
179:
175:
171:
168:
164:
160:
156:
152:
142:
140:
136:
132:
128:
124:
120:
116:
107:
104:
101:
97:
94:
90:
87:
83:
80:
76:
73:
70:
66:
63:
60:
56:
52:
48:
44:
31:
27:
20:
441:
437:
431:
406:
402:
396:
385:. Retrieved
381:
354:. Retrieved
343:The Guardian
342:
314:. Retrieved
310:
279:. Retrieved
277:(in Italian)
274:
240:Philadelphia
233:
220:
205:
193:
148:
114:
113:
99:Institutions
89:Biochemistry
78:
43:Milan, Italy
526:1949 births
224:brain drain
131:extract RNA
50:Nationality
480:Categories
387:2020-02-29
356:2020-02-28
316:2020-02-28
281:2020-02-29
254:References
129:method to
35:1949-09-20
458:1750-2799
423:0003-2697
351:0261-3077
244:President
230:Accolades
216:epigenome
170:Rotterdam
466:17406285
200:leukemia
159:genetics
119:oncology
93:Oncology
187:at the
176:by the
121:at the
53:Italian
464:
456:
421:
349:
145:Career
135:Trizol
85:Fields
163:yeast
151:Milan
139:cited
462:PMID
454:ISSN
419:ISSN
347:ISSN
29:Born
446:doi
411:doi
407:162
238:in
210:at
178:TdT
161:of
482::
460:.
452:.
440:.
417:.
405:.
380:.
365:^
345:.
341:.
325:^
309:.
290:^
273:.
262:^
250:.
191:.
91:,
468:.
448::
442:1
425:.
413::
390:.
359:.
319:.
284:.
37:)
33:(
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.