54:
Historically, pressure jumps were limited to one direction. Most commonly fast drops in pressure were achieved by using a quick release valve or a fast burst membrane. Modern equipment can achieve pressure changes in both directions using either double reservoir arrangements (good for large changes
505:
Malnási-Csizmadia, A; Pearson, D.S.; Kovács, M.; Woolley, R.J.; Geeves, M.A.; Bagshaw, C.R. (2001). "Kinetic
Resolution of a Conformational Transition and the ATP Hydrolysis Step Using Relaxation Methods with a Dictyostelium Myosin II Mutant Containing a Single Tryptophan Residue".
59:(often faster than valve based approaches). Ultra fast pressure drops can be achieved using electrically disintegrated burst membranes. The ability to automatically repeat measurements and average the results is useful since the reaction amplitudes are often small.
162:
423:"A novel pressure jump apparatus for the microvolume analysis of protein-ligand and protein-protein interactions: its application to nucleotide binding to skeletal-muscle and smooth-muscle myosin subfragment 1"
198:(1/Ď„) equal to the sum of the forward and reverse intrinsic rate constants. In more complex reaction networks, when multiple reaction steps are perturbed, then the reciprocal time constants are given by the
31:. This allows the study of the shift in equilibrium of reactions that equilibrate in periods between milliseconds to hours (or longer), these changes often being observed using
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of the characteristic rate equations. The ability to observe intermediate steps in a reaction pathway is one of the attractive features of this technology.
84:
318:
Heuer U, Krumova M, Hempel G, Schiewek M, Blume A (2010). "NMR probe for pressure-jump experiments up to 250 bars and 3 ms jump time".
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Schiewek M, Krumova M, Hempel G, Blume A (2007). "Pressure jump relaxation setup with IR detection and millisecond time resolution".
386:
Marchal S, Font J, RibĂł M, Vilanova M, Phillips RS, Lange R, Torrent J (2009). "Asymmetric kinetics of protein structural changes".
44:
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Dumont C, Emilsson T, Gruebele M (2009). "Reaching the protein folding speed limit with large, sub-microsecond pressure jumps".
363:
Methods for studying fast kinetics in biological systems in: Davies DB, Saenger W, Danyluk SS (Eds) Structural
Molecular Biology
370:
232:
Gruenewald B, Knoche W (1978). "Pressure jump method with detection of optical-rotation and circular-dichroism".
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When a single step in a reaction is perturbed in a pressure jump experiment, the reaction follows a single
23:. It involves making rapid changes to the pressure of an experimental system and observing the return to
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the percentage change in concentration of a measurable species) depends on the molar volume change (Δ
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157:{\displaystyle \Delta V^{o}=-RT\left({\frac {\partial \ln K}{\partial P}}\right)_{T}}
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Pearson DS, Holtermann G, Ellison P, Cremo C, Geeves MA (2002).
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is the pressure then the volume change is given by:
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320:Review of Scientific Instruments
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234:Review of Scientific Instruments
74:is the equilibrium constant and
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187:associated with the reaction.
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388:Accounts of Chemical Research
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194:function with the reciprocal
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365:. Plenum Publishing Corp.
37:fluorescence spectroscopy
57:piezoelectric actuators
33:absorption spectroscopy
173:universal gas constant
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218:This contrasts with
181:absolute temperature
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427:Biochemical Journal
361:Pörschke D (1982).
332:2010RScI...81j5102H
289:2007RScI...78d5101S
246:1978RScI...49..797G
484:10.1038/nmeth.1336
439:10.1042/BJ20020462
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51:can also be used.
548:Chemical kinetics
520:10.1021/bi010963q
400:10.1021/ar800266r
340:10.1063/1.3481164
297:10.1063/1.2719020
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192:exponential decay
185:Gibbs free energy
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200:eigenvalues
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206:References
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