539:
543:
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Sephadex is used to separate molecules by molecular weight. Sephadex is a faster alternative to dialysis (de-salting), requiring a low dilution factor (as little as 1.4:1), with high activity recoveries. Sephadex is also used for buffer exchange and the removal of small molecules during the
68:
media are composed of macroscopic beads synthetically derived from the polysaccharide dextran. The organic chains are cross-linked to give a three-dimensional network having functional ionic groups attached by ether linkages to glucose units of the polysaccharide chains.
98:
A special hydroxypropylated form of
Sephadex resin, named Sephadex LH-20, is used for the separation and purification of small organic molecules such as steroids, terpenoids, lipids. An example of use is the purification of cholesterol.
72:
Available forms include anion and cation exchangers, as well as gel filtration resins, with varying degrees of porosity; bead sizes fall in discrete ranges between 20 and 300
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427:
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preparation of large biomolecules, such as ampholytes, detergents, radioactive or fluorescent labels, and phenol (during DNA purification).
42:
435:
659:
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58:
30:
624:
61:
columns. By varying the degree of cross-linking, the fractionation properties of the gel can be altered.
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664:
518:
457:
Porath, J; Flodin, P (1959). "Gel
Filtration: A Method for Desalting and Group Separation".
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8:
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38:
587:"Pregnenolone separation from cholesterol using Sephadex LH-20 mini-columns"
57:
tran. It is normally manufactured in a bead form and most commonly used for
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20:
480:
407:
34:
586:
402:
23:
540:"GE Healthcare Life Sciences - Instructions for Sephadex Media"
112:
Fractionation Range of
Globular Proteins and Dextrans (Da).
26:
79:
Sephadex is also used for ion-exchange chromatography.
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651:
578:
634:(product manual). GE Healthcare. Apr 7, 2008
456:
64:These highly specialized gel filtration and
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652:
542:. .gelifesciences.com. Archived from
37:in 1959, after development work by
13:
14:
681:
625:"Instructions for Sephadex Media"
585:Hanukoglu I, Jefcoate CR (1980).
428:"From Sephadex to GE Healthcare"
102:
89:
617:
557:
532:
519:"Sephadex® ion exchange media"
511:
450:
420:
1:
603:10.1016/S0021-9673(00)85545-4
413:
398:Size exclusion chromatography
82:Sephadex is crosslinked with
271:Ion-exchange chromatography.
7:
591:Journal of Chromatography A
386:
45:. The name is derived from
10:
686:
120:
117:
108:Exclusion chromatography.
355:quaternary aminoethyl
344:quaternary aminoethyl
333:2-(diethylamino)ethyl
322:2-(diethylamino)ethyl
33:. It was launched by
660:Biochemistry methods
632:gelifesciences.co.jp
528:. Pharmacia Biotech.
121:Fractionation Range
526:chembio.uoguelph.ca
473:1959Natur.183.1657P
467:(4676): 1657–1659.
330:Sephadex-DEAE A-50
319:Sephadex-DEAE A-25
276:
352:Sephadex-QAE A-50
341:Sephadex-QAE A-25
275:
481:10.1038/1831657a0
384:
383:
374:Sephadex-SP C-50
363:Sephadex-SP C-25
308:Sephadex-CM C-50
297:Sephadex-CM C-25
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566:"Sephadex LH-20"
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438:on July 31, 2012
434:. Archived from
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564:GE Healthcare.
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84:epichlorohydrin
66:chromatographic
12:
11:
5:
683:
673:
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670:Swedish brands
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665:Chromatography
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597:(1): 256–262.
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432:chemeurope.com
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311:carboxymethyl
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300:carboxymethyl
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59:gel filtration
31:gel filtration
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546:on 2012-02-18
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291:Bead size (μ)
289:
287:
286:Functionality
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279:
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273:
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264:1000–200,000
263:
261:5000–250,000
260:
257:
256:
253:1000–200,000
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250:5000–600,000
249:
246:
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242:1000–150,000
241:
239:5000–150,000
238:
235:
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231:1000–150,000
230:
228:5000–300,000
227:
224:
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220:1000–100,000
219:
217:4000–100,000
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209:1000–100,000
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206:4000–150,000
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103:Fractionation
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39:Jerker Porath
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636:. Retrieved
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619:
594:
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569:. Retrieved
559:
548:. Retrieved
544:the original
534:
525:
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464:
458:
452:
440:. Retrieved
436:the original
431:
422:
377:sulfopropyl
366:sulfopropyl
290:
285:
280:
270:
269:
198:1000–50,000
195:3000–70,000
187:1000–50,000
184:3000–80,000
173:1500–30,000
127:
111:
107:
106:
97:
93:
90:Applications
81:
78:
71:
63:
54:
50:
46:
21:cross-linked
16:
15:
281:Description
176:500–10,000
654:Categories
571:2019-12-06
550:2011-12-02
414:References
393:PEGylation
165:100–5,000
162:1000–5000
43:Per Flodin
611:0021-9673
489:1476-4687
408:Sepharose
258:G-200 SF
236:G-150 SF
214:G-100 SF
132:Dextrans
128:Proteins
126:Globular
118:Gel Type
49:paration
35:Pharmacia
29:used for
505:32287460
497:13666849
403:Superose
387:See also
192:G-75 SF
17:Sephadex
469:Bibcode
380:40-120
369:40-120
358:40-120
347:40-120
336:40-120
325:40-120
314:40-120
303:40-120
53:rmacia
24:dextran
638:Jan 2,
609:
503:
495:
487:
460:Nature
442:Jan 2,
154:≤1500
151:≤1500
628:(PDF)
522:(PDF)
501:S2CID
247:G-200
225:G-150
203:G-100
143:≤700
140:≤700
19:is a
640:2019
607:ISSN
493:PMID
485:ISSN
444:2019
181:G-75
170:G-50
159:G-25
148:G-15
137:G-10
41:and
599:doi
595:190
477:doi
465:183
55:dex
51:Pha
27:gel
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605:.
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463:.
430:.
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74:μm
47:se
642:.
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446:.
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.