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Various grades and chemistries of sepharose are available. Iodoacetyl functional groups can be added to selectively bind
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65:. Sepharose/agarose, combined with some form of activation chemistry, is also used to immobilize
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Principles and
Reactions of Protein Extraction, Purification, and Characterization
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to attach proteins to the agarose resin through lysine side chains.
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attachment to the resin. Common activation chemistries include
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side chains and this method is often used to immobilize
19:is a tradename for a crosslinked, beaded-form of
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99:Gel permeation chromatography
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128:Ahmed, Hafiz (2004).
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