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to form a gel-like mass. The pores in this material have different sizes, and if a molecule is too big, it does not fit into the pores, meaning that it follows a shorter way to the end of the column.
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Lee SC, Whitaker JR (August 2004). "Are molecular weights of proteins determined by superose 12 column chromatography correct?".
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which are used in the automated separation of biological molecules. The different columns provided can separate a variety of
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Andersson, T et al. Agarose-based media for high-resolution gel filtration of biopolymers. J. Chromatogr. 326, 33 (1985).
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Dubin PL, Principi JM (September 1989). "Optimization of size-exclusion separation of proteins on a
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The columns are placed in a holder, and a computerized pumping system pumps a watery solution, often a
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