17:
1803:
1783:
293:
The ATPase domain is essential during the interactions of the proteins Hsp70 and subunit Tim44. Without the presence of ATPase, carboxy-terminal segment is not able to bind to protein of Tim44. As mtHsp70 transmits the nucleotide state of the ATPase domain with alpha-helices A and B, Tim44 interacts
20:
Simplified representation of the
Mitochondrial DNA Organization proteins (top image). A close up of a single ribosome in coordination with the TOM complex on the outer Mitochondrial membrane and the TIM complex on the inner Mitochondrial membrane (bottom image). The nascent transmembrane protein is
286:(TIM) complex, which are hypothesized to be transiently linked at sites of close contact between the two membranes. The signal sequence is then translocated into the matrix in a process that requires an electrochemical hydrogen ion gradient across the inner membrane. Mitochondrial
306:
located on the inner and outer membranes of the chloroplast, in the sense that it transports proteins into the membrane of the mitochondria. Although they both hydrolyze triphosphates, they are evolutionally unrelated.
341:"Mitochondrial protein import motor: the ATPase domain of matrix Hsp70 is crucial for binding to Tim44, while the peptide binding domain and the carboxy-terminal segment play a stimulatory role"
176:
610:
1444:
501:"The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70/ATP driving system"
195:
270:
and synthesised in the cytoplasm. These are tagged by an N-terminal or/and a C-terminal signal sequence. Following transport through the
1303:
868:
858:
812:
887:
603:
188:
833:
1888:
1844:
139:
802:
596:
1502:
283:
275:
1873:
1393:
534:
Voos W, Martin H, Krimmer T, Pfanner N (November 1999). "Mechanisms of protein translocation into mitochondria".
458:"Multiple interactions of components mediating preprotein translocation across the inner mitochondrial membrane"
1388:
133:
115:
1658:
1410:
290:
binds to regions of the polypeptide chain and maintains it in an unfolded state as it moves into the matrix.
120:
1458:
980:
775:
703:
699:
676:
636:
1863:
1380:
200:
1773:
108:
578:
398:"Mitochondrial protein import: molecular basis of the ATP-dependent interaction of MtHsp70 with Tim44"
1837:
1759:
1746:
1733:
1720:
1707:
1694:
1681:
1643:
1439:
878:
649:
623:
584:
229:
1653:
1607:
1550:
994:
689:
654:
627:
241:
38:
136:
1555:
1364:
1327:
666:
588:
60:
396:
Moro, Fernando; Okamoto, Koji; Donzeau, Mariel; Neupert, Walter; Brunner, Michael (2002-03-01).
1094:
734:
252:
1893:
1868:
1576:
1495:
1224:
1132:
499:
Berthold J, Bauer MF, Schneider HC, Klaus C, Dietmeier K, Neupert W, Brunner M (June 1995).
21:
being fed into the mitochondrial membrane where its target peptide (not shown) gets cleaved.
1830:
1648:
96:
8:
1818:
1612:
1429:
16:
72:
1545:
1010:
482:
457:
43:
547:
517:
500:
456:
Bömer U, Meijer M, Maarse AC, Hönlinger A, Dekker PJ, Pfanner N, Rassow J (May 1997).
151:
1883:
1878:
684:
551:
522:
487:
427:
419:
378:
373:
360:
356:
340:
263:
127:
1591:
1586:
1560:
1488:
1074:
1020:
1002:
543:
512:
477:
469:
409:
368:
352:
266:. The vast majority of proteins destined for the mitochondria are encoded in the
1638:
1622:
1535:
1421:
1310:
1124:
824:
644:
572:
303:
274:
from the nucleus, the signal sequence is recognized by a receptor protein in the
155:
1814:
1787:
1676:
1617:
744:
171:
473:
1857:
1802:
1581:
1540:
1288:
898:
423:
364:
259:
146:
1530:
1273:
1246:
1175:
1168:
1151:
1146:
1120:
555:
431:
414:
397:
382:
339:
Krimmer, T.; Rassow, J.; Kunau, W. H.; Voos, W.; Pfanner, N. (2000-08-01).
267:
217:
526:
491:
1754:
1689:
1525:
1369:
279:
1354:
1043:
244:
233:
84:
1728:
1702:
1060:
619:
282:
chain are inserted in the TOM complex, then begin interaction with a
1782:
323:
B. Alberts, A. Johnson, J. Lewis, M. Raff,. K. Roberts, P. Walter.
221:
618:
1465:
1434:
1212:
1207:
966:
956:
931:
926:
661:
278:(TOM) complex. The signal sequence and adjacent portions of the
271:
103:
294:
with the peptide binding domain to coordinate the protein bind.
1810:
1741:
1511:
1342:
984:
961:
951:
921:
916:
911:
906:
863:
853:
848:
843:
838:
807:
795:
790:
785:
780:
768:
763:
758:
707:
183:
79:
67:
55:
1715:
1398:
1337:
1320:
1315:
1163:
1141:
1107:
1102:
1087:
1065:
1053:
1048:
1038:
1033:
1028:
753:
729:
536:
Biochimica et
Biophysica Acta (BBA) - Reviews on Biomembranes
287:
1403:
1359:
1347:
1332:
1298:
1293:
1278:
1261:
1256:
1251:
1239:
1234:
1229:
1219:
1195:
1190:
1185:
1180:
1082:
569:
498:
91:
1480:
455:
1453:
1449:
395:
225:
533:
338:
302:
This protein complex is functionally analogous to the
297:
249:
ATP phosphohydrolase (mitochondrial protein-importing)
1771:
579:
Overview of the various import ways into mitochondria
1855:
228:through the mitochondrial membrane for use in
1838:
1496:
604:
1845:
1831:
1503:
1489:
611:
597:
516:
481:
413:
372:
238:mitochondrial protein-transporting ATPase
28:mitochondrial protein-transporting ATPase
15:
446:Toc, Tic and chloroplast protein import
1856:
1484:
592:
1797:
334:
332:
575:- description of the entire complex
402:The Journal of Biological Chemistry
298:TIC/TOC Complex vs. TIM/TOM Complex
13:
14:
1905:
563:
329:
284:translocase of the inner membrane
276:translocase of the outer membrane
258:Only 13 proteins necessary for a
236:, the complex is described as an
216:is a protein complex in cellular
1801:
1781:
357:10.1128/mcb.20.16.5879-5887.2000
438:
389:
345:Molecular and Cellular Biology
317:
1:
548:10.1016/s0304-4157(99)00007-6
518:10.1016/S0092-8674(05)80013-3
325:Molecular Biology of the Cell
310:
1889:Enzymes of unknown structure
1817:. You can help Knowledge by
7:
1510:
1381:Protein-synthesizing GTPase
251:, as the TIM part requires
10:
1910:
1796:
247:), or more systematically
1667:
1659:MichaelisâMenten kinetics
1631:
1600:
1569:
1518:
1440:Guanylate-binding protein
1419:
1378:
1118:
992:
979:
944:
896:
877:
823:
743:
722:
715:
698:
675:
635:
624:acid anhydride hydrolases
230:oxidative phosphorylation
194:
182:
170:
165:
161:
145:
126:
114:
102:
90:
78:
66:
54:
49:
37:
32:
27:
1551:Diffusion-limited enzyme
995:Heterotrimeric G protein
690:Phosphoadenylylsulfatase
667:Thiamine-triphosphatase
474:10.1093/emboj/16.9.2205
1874:Transmembrane proteins
1813:-related article is a
415:10.1074/jbc.M107935200
262:are actually coded in
224:produced from nuclear
22:
1644:EadieâHofstee diagram
1577:Allosteric regulation
1422:Polymerization motors
1133:Rho family of GTPases
581:(group of N. Pfanner)
19:
1654:LineweaverâBurk plot
444:P. Jarvis, J. Soll,
255:hydrolysis to work.
1430:dynamin superfamily
220:which translocates
1864:Transport proteins
1613:Enzyme superfamily
1546:Enzyme promiscuity
23:
1826:
1825:
1769:
1768:
1478:
1477:
1474:
1473:
975:
974:
940:
939:
685:Adenylylsulfatase
351:(16): 5879â5887.
264:mitochondrial DNA
210:
209:
206:
205:
109:metabolic pathway
1901:
1847:
1840:
1833:
1805:
1798:
1786:
1785:
1777:
1649:HanesâWoolf plot
1592:Enzyme activator
1587:Enzyme inhibitor
1561:Enzyme catalysis
1505:
1498:
1491:
1482:
1481:
990:
989:
720:
719:
713:
712:
613:
606:
599:
590:
589:
559:
530:
520:
495:
485:
462:The EMBO Journal
448:
442:
436:
435:
417:
408:(9): 6874â6880.
393:
387:
386:
376:
336:
327:
321:
163:
162:
25:
24:
1909:
1908:
1904:
1903:
1902:
1900:
1899:
1898:
1854:
1853:
1852:
1851:
1794:
1792:
1780:
1772:
1770:
1765:
1677:Oxidoreductases
1663:
1639:Enzyme kinetics
1627:
1623:List of enzymes
1596:
1565:
1536:Catalytic triad
1514:
1509:
1479:
1470:
1415:
1374:
1125:Ras superfamily
1114:
1098:
1078:
1024:
1014:
1006:
971:
936:
892:
873:
819:
776:Plasma membrane
739:
694:
671:
645:Pyrophosphatase
631:
617:
566:
452:
451:
443:
439:
394:
390:
337:
330:
322:
318:
313:
304:TIC/TOC complex
300:
214:TIM/TOM complex
12:
11:
5:
1907:
1897:
1896:
1891:
1886:
1881:
1876:
1871:
1866:
1850:
1849:
1842:
1835:
1827:
1824:
1823:
1806:
1791:
1790:
1767:
1766:
1764:
1763:
1750:
1737:
1724:
1711:
1698:
1685:
1671:
1669:
1665:
1664:
1662:
1661:
1656:
1651:
1646:
1641:
1635:
1633:
1629:
1628:
1626:
1625:
1620:
1615:
1610:
1604:
1602:
1601:Classification
1598:
1597:
1595:
1594:
1589:
1584:
1579:
1573:
1571:
1567:
1566:
1564:
1563:
1558:
1553:
1548:
1543:
1538:
1533:
1528:
1522:
1520:
1516:
1515:
1508:
1507:
1500:
1493:
1485:
1476:
1475:
1472:
1471:
1469:
1468:
1463:
1462:
1461:
1456:
1447:
1442:
1437:
1426:
1424:
1417:
1416:
1414:
1413:
1408:
1407:
1406:
1401:
1396:
1385:
1383:
1376:
1375:
1373:
1372:
1367:
1362:
1357:
1352:
1351:
1350:
1345:
1340:
1335:
1325:
1324:
1323:
1318:
1308:
1307:
1306:
1301:
1296:
1284:
1283:
1282:
1281:
1276:
1266:
1265:
1264:
1259:
1254:
1244:
1243:
1242:
1237:
1232:
1222:
1217:
1216:
1215:
1210:
1200:
1199:
1198:
1193:
1188:
1183:
1173:
1172:
1171:
1166:
1156:
1155:
1154:
1149:
1144:
1129:
1127:
1116:
1115:
1113:
1112:
1111:
1110:
1105:
1096:
1092:
1091:
1090:
1085:
1076:
1072:
1071:
1070:
1069:
1068:
1058:
1057:
1056:
1051:
1041:
1036:
1031:
1022:
1018:
1017:
1016:
1012:
1004:
999:
997:
987:
977:
976:
973:
972:
970:
969:
964:
959:
954:
948:
946:
942:
941:
938:
937:
935:
934:
929:
924:
919:
914:
909:
903:
901:
894:
893:
891:
890:
884:
882:
875:
874:
872:
871:
866:
861:
856:
851:
846:
841:
836:
830:
828:
821:
820:
818:
817:
816:
815:
810:
800:
799:
798:
793:
788:
783:
773:
772:
771:
766:
761:
750:
748:
741:
740:
738:
737:
732:
726:
724:
723:Cu++ (3.6.3.4)
717:
710:
696:
695:
693:
692:
687:
681:
679:
673:
672:
670:
669:
664:
659:
658:
657:
652:
641:
639:
633:
632:
616:
615:
608:
601:
593:
587:
586:
582:
576:
565:
564:External links
562:
561:
560:
531:
511:(7): 1085â93.
496:
468:(9): 2205â16.
450:
449:
437:
388:
328:
315:
314:
312:
309:
299:
296:
208:
207:
204:
203:
198:
192:
191:
186:
180:
179:
174:
168:
167:
159:
158:
149:
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131:
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123:
118:
112:
111:
106:
100:
99:
94:
88:
87:
82:
76:
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70:
64:
63:
58:
52:
51:
47:
46:
41:
35:
34:
30:
29:
9:
6:
4:
3:
2:
1906:
1895:
1892:
1890:
1887:
1885:
1882:
1880:
1877:
1875:
1872:
1870:
1867:
1865:
1862:
1861:
1859:
1848:
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1841:
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1829:
1828:
1822:
1820:
1816:
1812:
1807:
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1799:
1795:
1789:
1784:
1779:
1778:
1775:
1761:
1757:
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1751:
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1731:
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1725:
1722:
1718:
1717:
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1704:
1699:
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1686:
1683:
1679:
1678:
1673:
1672:
1670:
1666:
1660:
1657:
1655:
1652:
1650:
1647:
1645:
1642:
1640:
1637:
1636:
1634:
1630:
1624:
1621:
1619:
1618:Enzyme family
1616:
1614:
1611:
1609:
1606:
1605:
1603:
1599:
1593:
1590:
1588:
1585:
1583:
1582:Cooperativity
1580:
1578:
1575:
1574:
1572:
1568:
1562:
1559:
1557:
1554:
1552:
1549:
1547:
1544:
1542:
1541:Oxyanion hole
1539:
1537:
1534:
1532:
1529:
1527:
1524:
1523:
1521:
1517:
1513:
1506:
1501:
1499:
1494:
1492:
1487:
1486:
1483:
1467:
1464:
1460:
1457:
1455:
1451:
1448:
1446:
1443:
1441:
1438:
1436:
1433:
1432:
1431:
1428:
1427:
1425:
1423:
1418:
1412:
1409:
1405:
1402:
1400:
1397:
1395:
1392:
1391:
1390:
1387:
1386:
1384:
1382:
1377:
1371:
1368:
1366:
1363:
1361:
1358:
1356:
1353:
1349:
1346:
1344:
1341:
1339:
1336:
1334:
1331:
1330:
1329:
1326:
1322:
1319:
1317:
1314:
1313:
1312:
1309:
1305:
1302:
1300:
1297:
1295:
1292:
1291:
1290:
1286:
1285:
1280:
1277:
1275:
1272:
1271:
1270:
1267:
1263:
1260:
1258:
1255:
1253:
1250:
1249:
1248:
1245:
1241:
1238:
1236:
1233:
1231:
1228:
1227:
1226:
1223:
1221:
1218:
1214:
1211:
1209:
1206:
1205:
1204:
1201:
1197:
1194:
1192:
1189:
1187:
1184:
1182:
1179:
1178:
1177:
1174:
1170:
1167:
1165:
1162:
1161:
1160:
1157:
1153:
1150:
1148:
1145:
1143:
1140:
1139:
1138:
1134:
1131:
1130:
1128:
1126:
1122:
1117:
1109:
1106:
1104:
1101:
1100:
1099:
1093:
1089:
1086:
1084:
1081:
1080:
1079:
1073:
1067:
1064:
1063:
1062:
1059:
1055:
1052:
1050:
1047:
1046:
1045:
1042:
1040:
1037:
1035:
1032:
1030:
1027:
1026:
1025:
1019:
1015:
1009:
1008:
1007:
1001:
1000:
998:
996:
991:
988:
986:
982:
978:
968:
965:
963:
960:
958:
955:
953:
950:
949:
947:
943:
933:
930:
928:
925:
923:
920:
918:
915:
913:
910:
908:
905:
904:
902:
900:
899:P-type ATPase
895:
889:
886:
885:
883:
880:
876:
870:
867:
865:
862:
860:
857:
855:
852:
850:
847:
845:
842:
840:
837:
835:
832:
831:
829:
826:
822:
814:
811:
809:
806:
805:
804:
801:
797:
794:
792:
789:
787:
784:
782:
779:
778:
777:
774:
770:
767:
765:
762:
760:
757:
756:
755:
752:
751:
749:
746:
742:
736:
733:
731:
728:
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725:
721:
718:
714:
711:
709:
705:
701:
697:
691:
688:
686:
683:
682:
680:
678:
674:
668:
665:
663:
660:
656:
653:
651:
648:
647:
646:
643:
642:
640:
638:
634:
629:
625:
621:
614:
609:
607:
602:
600:
595:
594:
591:
585:
583:
580:
577:
574:
571:
568:
567:
557:
553:
549:
545:
542:(3): 235â54.
541:
537:
532:
528:
524:
519:
514:
510:
506:
502:
497:
493:
489:
484:
479:
475:
471:
467:
463:
459:
454:
453:
447:
441:
433:
429:
425:
421:
416:
411:
407:
403:
399:
392:
384:
380:
375:
370:
366:
362:
358:
354:
350:
346:
342:
335:
333:
326:
320:
316:
308:
305:
295:
291:
289:
285:
281:
277:
273:
269:
265:
261:
260:mitochondrion
256:
254:
250:
246:
243:
239:
235:
231:
227:
223:
219:
215:
202:
199:
197:
193:
190:
187:
185:
181:
178:
175:
173:
169:
164:
160:
157:
153:
150:
148:
147:Gene Ontology
144:
141:
138:
135:
132:
129:
125:
122:
119:
117:
113:
110:
107:
105:
101:
98:
95:
93:
89:
86:
85:NiceZyme view
83:
81:
77:
74:
71:
69:
65:
62:
59:
57:
53:
48:
45:
42:
40:
36:
31:
26:
18:
1894:Enzyme stubs
1869:Mitochondria
1819:expanding it
1808:
1793:
1755:Translocases
1752:
1739:
1726:
1713:
1700:
1690:Transferases
1687:
1674:
1531:Binding site
1268:
1202:
1158:
1136:
1121:Small GTPase
735:Wilson/ATP7B
730:Menkes/ATP7A
539:
535:
508:
504:
465:
461:
445:
440:
405:
401:
391:
348:
344:
324:
319:
301:
292:
257:
248:
237:
218:biochemistry
213:
211:
73:BRENDA entry
1526:Active site
1445:Mitofusin-1
1420:3.6.5.5-6:
1389:Prokaryotic
280:polypeptide
61:IntEnz view
33:Identifiers
1858:Categories
1729:Isomerases
1703:Hydrolases
1570:Regulation
1411:Eukaryotic
1044:Transducin
881:(3.6.3.10)
620:Hydrolases
311:References
234:enzymology
130:structures
97:KEGG entry
1608:EC number
1379:3.6.5.3:
1119:3.6.5.2:
1061:Gustducin
993:3.6.5.1:
827:(3.6.3.9)
747:(3.6.3.8)
650:Inorganic
424:0021-9258
365:0270-7306
50:Databases
1884:EC 7.4.2
1879:EC 3.6.3
1632:Kinetics
1556:Cofactor
1519:Activity
655:Thiamine
556:10548718
432:11733493
383:10913171
222:proteins
201:proteins
189:articles
177:articles
134:RCSB PDB
1788:Biology
1742:Ligases
1512:Enzymes
1466:Tubulin
1435:Dynamin
1287:other:
967:Katanin
957:Kinesin
932:ATP13A3
927:ATP13A2
662:Apyrase
527:7600576
492:9171336
483:1169823
272:cytosol
268:nucleus
245:7.4.2.3
156:QuickGO
121:profile
104:MetaCyc
44:7.4.2.3
1811:enzyme
1774:Portal
1716:Lyases
1343:ARL13B
1203:RhoBTB
1097:α12/13
985:GTPase
962:Myosin
952:Dynein
922:ATP12A
917:ATP11B
912:ATP10A
907:ATP8B1
897:Other
869:ATP1B4
864:ATP1B3
859:ATP1B2
854:ATP1B1
849:ATP1A4
844:ATP1A3
839:ATP1A2
834:ATP1A1
825:Na+/K+
813:ATP2C2
808:ATP2C1
796:ATP2B4
791:ATP2B3
786:ATP2B2
781:ATP2B1
769:ATP2A3
764:ATP2A2
759:ATP2A1
708:ATPase
554:
525:
490:
480:
430:
422:
381:
371:
363:
184:PubMed
166:Search
152:AmiGO
140:PDBsum
80:ExPASy
68:BRENDA
56:IntEnz
39:EC no.
1809:This
1668:Types
1399:EF-Tu
1338:SAR1B
1321:RAB27
1316:RAB23
1269:RhoDF
1159:RhoUV
1142:CDC42
1137:Cdc42
1123:>
1108:GNA13
1103:GNA12
1088:GNA11
1077:αq/11
1066:GNAT3
1054:GNAT2
1049:GNAT1
1039:GNAI3
1034:GNAI2
1029:GNAI1
981:3.6.5
945:3.6.4
888:ATP4A
879:H+/K+
754:SERCA
716:3.6.3
700:3.6.3
677:3.6.2
637:3.6.1
573:3.A.8
374:86065
288:Hsp70
232:. In
116:PRIAM
1815:stub
1760:list
1753:EC7
1747:list
1740:EC6
1734:list
1727:EC5
1721:list
1714:EC4
1708:list
1701:EC3
1695:list
1688:EC2
1682:list
1675:EC1
1459:OPA1
1452:and
1404:EF-G
1394:IF-2
1360:Rheb
1348:ARL6
1333:ARF6
1304:NRAS
1299:KRAS
1294:HRAS
1279:RhoD
1274:RhoF
1220:RhoH
1196:RhoG
1181:Rac1
1169:RhoV
1164:RhoU
1147:TC10
1083:GNAQ
803:SPCA
630:3.6)
570:TCDB
552:PMID
540:1422
523:PMID
505:Cell
488:PMID
428:PMID
420:ISSN
379:PMID
361:ISSN
212:The
196:NCBI
137:PDBe
92:KEGG
1454:MX2
1450:MX1
1370:RGK
1365:Rap
1355:Ran
1328:Arf
1311:Rab
1289:Ras
1247:Rnd
1225:Rho
1176:Rac
1152:TCL
1013:olf
745:Ca+
544:doi
513:doi
478:PMC
470:doi
410:doi
406:277
369:PMC
353:doi
253:ATP
226:DNA
172:PMC
128:PDB
1860::
1135::
1023:αi
1005:αs
983::
706::
628:EC
622::
550:.
538:.
521:.
509:81
507:.
503:.
486:.
476:.
466:16
464:.
460:.
426:.
418:.
404:.
400:.
377:.
367:.
359:.
349:20
347:.
343:.
331:^
242:EC
154:/
1846:e
1839:t
1832:v
1821:.
1776::
1762:)
1758:(
1749:)
1745:(
1736:)
1732:(
1723:)
1719:(
1710:)
1706:(
1697:)
1693:(
1684:)
1680:(
1504:e
1497:t
1490:v
1262:3
1257:2
1252:1
1240:C
1235:B
1230:A
1213:2
1208:1
1191:3
1186:2
1095:G
1075:G
1021:G
1011:G
1003:G
704:4
702:-
626:(
612:e
605:t
598:v
558:.
546::
529:.
515::
494:.
472::
434:.
412::
385:.
355::
240:(
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