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686:(PBS) on ice for 10 minutes. In histology and pathology specimens preparation, usually, the fixation step is performed using 10% Neutral Buffered Formalin (4% formaldehyde) for, at least, 24 hours.
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Paraformaldehyde is not a fixative; it must be depolymerized to formaldehyde in solution. In cell culture, a typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in
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with a typical degree of polymerization of 8–100 units. Paraformaldehyde commonly has a slight odor of formaldehyde due to decomposition. Paraformaldehyde is a poly-
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Cogliano, Vincent; Grosse, Yann; Baan, Robert; Straif, Kurt; Secretan, Béatrice; Ghissassi, Fatiha El (September 2004). "Advice on formaldehyde and glycol ethers".
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Paraformaldehyde can be used as a substitute of aqueous formaldehyde to produce the resinous binding material, which is commonly used together with
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in the presence of a base, an acid or heat. The high purity formaldehyde solutions obtained in this way are used as a
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Yates, J (1973). "Adsorption and decomposition of formaldehyde on tungsten (100) and (111) crystal planes".
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actually contains very little monomeric formaldehyde; most of it forms short chains of
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Once paraformaldehyde is depolymerized, the resulting formaldehyde may be used as a
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The resulting formaldehyde gas from dry heating paraformaldehyde is flammable.
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663:. Longer chain-length (high molecular weight) polyoxymethylenes are used as a
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Paraformaldehyde is also used to crosslink proteins to DNA, as used in ChIP (
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solutions as a white precipitate, especially if stored in the cold.
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Except where otherwise noted, data are given for materials in their
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As a formaldehyde releasing agent, paraformaldehyde is a potential
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to formaldehyde gas by dry heating and to formaldehyde solution by
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675:). It was used in the past in the discredited Sargenti method of
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817:"Be Wary of Sargenti Root Canal Treatment | Quackwatch"
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744:(metaformaldehyde), the cyclic trimer of formaldehyde
880:"MSDS - 158127 SAFETY DATA SHEET - Paraformaldehyde"
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704:or other reactive agents in the manufacturing of
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225:White powder with formaldehyde-like odour
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584:Paraformaldehyde forms slowly in aqueous
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438:300 °C (572 °F; 573 K)
245:120 °C (248 °F; 393 K)
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424:71 °C (160 °F; 344 K)
831:"Paraformaldehyde - Dover Chemical"
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515:(at 25 °C , 100 kPa).
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1:
858:10.1016/S1470-2045(04)01562-1
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691:chromatin immunoprecipitation
803:10.1016/0021-9517(73)90073-0
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732:in rats is 592 mg/kg.
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456:or concentration (LD, LC):
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684:phosphate buffered saline
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616:Paraformaldehyde can be
16:Not to be confused with
642:
604:to limit the extent of
448:7.0% (low), 73% (high)
217:H (n = 8 - 100)
474:800 mg/kg (rat, oral)
388:
387:
790:Journal of Catalysis
677:root canal treatment
600:is often added as a
596:. A small amount of
492:1070 mg/m (rat, 4h)
486:median concentration
370:(fire diamond)
846:The Lancet Oncology
763:"C&L Inventory"
252:Solubility in water
25:
730:median lethal dose
560:) is the smallest
542:Infobox references
389:
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728:. Its acute oral
708:, medium density
667:and are known as
550:Chemical compound
548:
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504:Fisher Scientific
498:Safety data sheet
325:Hazard statements
257:slightly soluble
165:CompTox Dashboard
24:Paraformaldehyde
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562:polyoxymethylene
554:Paraformaldehyde
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882:. SIGMA-ALDRICH
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98:ECHA InfoCard
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884:. Retrieved
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770:. Retrieved
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653:disinfectant
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586:formaldehyde
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570:formaldehyde
557:
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431:Autoignition
366:
316:
278:
53:Identifiers
913:Hemiacetals
886:15 February
819:. May 2018.
772:27 December
568:product of
468:median dose
454:Lethal dose
433:temperature
420:Flash point
311:Signal word
222:Appearance
197:Properties
104:100.108.270
18:Paraldehyde
908:Polyethers
902:Categories
852:(9): 528.
797:(2): 260.
749:References
726:carcinogen
710:fiberboard
630:microscopy
602:stabilizer
290:Pictograms
267:1.579 kPa
235:1.46 g/cm
143:Y19UC83H8E
81:ChemSpider
70:30525-89-4
60:CAS Number
44:IUPAC name
657:fungicide
634:histology
612:Reactions
580:Synthesis
281:labelling
154:UN number
125:608-494-5
117:EC Number
866:15384217
736:See also
720:Toxicity
698:melamine
661:fixative
649:fumigant
626:fixative
598:methanol
590:Formalin
367:NFPA 704
272:Hazards
714:plywood
535:what is
533: (
231:Density
864:
702:phenol
673:Delrin
659:, and
574:acetal
564:, the
530:verify
527:
500:(SDS)
317:Danger
38:Names
622:water
209:OH(CH
158:2213
888:2022
862:PMID
774:2021
712:and
643:Uses
632:and
628:for
359:H351
355:H334
351:H332
347:H318
343:H317
339:H315
335:H302
331:H228
134:UNII
88:none
854:doi
799:doi
558:PFA
279:GHS
170:EPA
904::
860:.
848:.
795:30
793:.
765:.
716:.
700:,
679:.
655:,
651:,
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608:.
576:.
482:50
480:LC
464:50
462:LD
357:,
353:,
349:,
345:,
341:,
337:,
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283::
213:O)
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556:(
525:N
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408:1
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215:n
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168:(
20:.
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
