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162:, the consequences of which need to be taken into consideration when interpreting results. During recording, the tissue also "ages", degrading at a faster rate than in the intact animal. Finally, the artificial composition of the bathing solution means that the presence and relative concentrations of the necessary compounds may not be present.
152:
present in the whole brain. Further, the slicing process may itself compromise the tissue. To minimize complications in the slicing process, a more sophisticated tissue slicer may be used such as the
Compresstome, a type of vibrating microtome used to maximizes the amount of viable tissue cells.
86:
Free hand sectioning is a type of preparation techniques where a skilled operator uses razor blade for slicing. The blade is wetted with an isotonic solution before cutting to avoid tissue smudging during cutting. This method has several drawbacks such as sample size limitation and difficult to
123:
can be carefully adjusted and maintained. Slice work under a microscope also allows for careful placement of the recording electrode, which would not be possible in the closed in vivo system. Removing the brain tissue means that there is no
132:, or ions to be perfused throughout the neural tissue. Furthermore, the slice preparation method can also be used as a brain-injury model. Finally, whilst the circuit isolated in a brain slice represents a simplified model of the circuit
157:
and extraction of the brain before the slice is placed in solution may have effects on the tissue which are not yet understood. The slice preparation procedure itself induces a rapid and robust phenotype change in
35:
that allows the study of neurons from various brain regions in isolation from the rest of the brain, in an ex-vivo condition. Brain tissue is initially sliced via a tissue slicer then immersed in
99:
When investigating mammalian CNS activity, slice preparation has several advantages and disadvantages when compared to in vivo study. Slice preparation is both faster and cheaper than
1313:
67:. However, the increase in control comes with a decrease in the ease with which the results can be applied to the whole neural system.
439:
318:"Microglia contribute to neuronal synchrony despite endogenous ATP-related phenotypic transformation in acute mouse brain slices"
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467:
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beyond the initial sacrifice. The removal of the brain tissue from the body removes the mechanical effects of heartbeat and
484:
1293:
363:"Impact of variation in tissue preparation methodology on the functional outcome of neocortical mouse brain slices"
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Additionally, slicing of the brain can damage the top and bottom of the section, but beyond that, the process of
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683:
36:
1409:
1134:
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1109:
240:"A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system"
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devices such as
Compresstome microtomes are used to prepare slices as these devices have less limitations.
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994:
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855:
778:
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193:"Characteristics of CA1 neurons recorded intracellularly in the hippocampalin vitro slice preparation"
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Slice preparation also has some drawbacks. Most obviously, an isolated slice lacks the usual
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115:. The physiological conditions of the sample, such as oxygen and carbon dioxide levels, or
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39:(aCSF) for stimulation and/or recording. The technique allows for greater
1212:
1059:
642:
611:
591:
361:
Voss, Logan J.; Van Kan, Claudia; Envall, Gustav; Lamber, Oliver (2020).
136:, it maintains structural connections that are lost in cell cultures, or
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74:
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616:
255:
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64:
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48:
47:
of interest, careful control of the physiological conditions through
43:, through elimination of the effects of the rest of the brain on the
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479:
238:
Edwards, F. A.; Konnerth, A.; Sakmann, B.; Takahashi, T. (1989).
133:
100:
60:
417:
763:
473:
237:
950:
316:
Peter, Berki; Csaba, Cserep; Zsuzsanna, Környei (2024).
296:"slice preparation in laboratory in lab - Google Search"
360:
116:
315:
128:, which allows drugs, neurotransmitters or their
1423:
81:
244:PflĂĽgers Archiv European Journal of Physiology
779:
433:
190:
1314:Intraoperative neurophysiological monitoring
408:Brain Slice Preparation in Electrophysiology
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772:
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426:
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69:
16:Laboratory method involving brain slices
311:
309:
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767:
421:
1404:
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172:Histology § Sample preparation
13:
103:preparation, and does not require
14:
1453:
1294:Development of the nervous system
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191:Schwartzkroin, Philip A. (1975).
1403:
1392:
1391:
949:
793:
745:
734:
733:
490:Central pattern generator (CPG)
684:Frog hearing and communication
379:10.1016/j.brainres.2020.147043
354:
288:
265:11858/00-001M-0000-002C-2F28-1
231:
184:
143:
59:activity through perfusion of
37:artificial cerebrospinal fluid
1:
1135:Social cognitive neuroscience
177:
55:, to precise manipulation of
1110:Molecular cellular cognition
209:10.1016/0006-8993(75)90817-3
150:input and output connections
111:, which allows for extended
82:Slice preparation techniques
7:
1329:Neurodevelopmental disorder
1304:Neural network (biological)
1299:Neural network (artificial)
165:
94:
10:
1458:
856:Computational neuroscience
689:Infrared sensing in snakes
674:Jamming avoidance response
330:10.1038/s41467-024-49773-1
51:of substrates through the
1387:
1324:Neurodegenerative disease
1281:
1168:Evolutionary neuroscience
1143:
1083:
958:
947:
819:
801:
729:
656:
635:
539:
455:
87:observe progress. Modern
1289:Brain–computer interface
1238:Neuromorphic engineering
1163:Educational neuroscience
1070:Nutritional neuroscience
975:Clinical neurophysiology
871:Integrative neuroscience
694:Caridoid escape reaction
1100:Behavioral neuroscience
547:Theodore Holmes Bullock
113:intracellular recording
1095:Affective neuroscience
876:Molecular neuroscience
831:Behavioral epigenetics
704:Surface wave detection
78:
1442:Laboratory techniques
1158:Cultural neuroscience
1153:Consumer neuroscience
995:Neurogastroenterology
851:Cellular neuroscience
520:Anti-Hebbian learning
322:Nature Communications
77:slices, schematically
73:
1130:Sensory neuroscience
970:Behavioral neurology
941:Systems neuroscience
597:Bernhard Hassenstein
530:Ultrasound avoidance
505:Fixed action pattern
468:Coincidence detector
41:experimental control
29:laboratory technique
1273:Social neuroscience
1173:Global neurosurgery
1050:Neurorehabilitation
1020:Neuro-ophthalmology
1005:Neurointensive care
836:Behavioral genetics
664:Animal echolocation
602:Werner E. Reichardt
552:Walter Heiligenberg
126:blood–brain barrier
121:extracellular fluid
1349:Neuroimmune system
1243:Neurophenomenology
1183:Neural engineering
906:Neuroendocrinology
886:Neural engineering
627:Fernando Nottebohm
525:Sound localization
500:Lateral inhibition
256:10.1007/BF00580998
138:homogenised tissue
79:
1437:Electrophysiology
1419:
1418:
1268:Paleoneurobiology
1203:Neuroepistemology
1178:Neuroanthropology
1144:Interdisciplinary
1030:Neuropharmacology
990:Neuroepidemiology
761:
760:
648:Slice preparation
510:Krogh's Principle
485:Feature detection
33:electrophysiology
21:slice preparation
1449:
1407:
1406:
1395:
1394:
1309:Detection theory
1193:Neurocriminology
1120:Neurolinguistics
1035:Neuroprosthetics
953:
916:Neuroinformatics
866:Imaging genetics
788:
781:
774:
765:
764:
749:
748:
737:
736:
714:Mechanoreception
709:Electroreception
622:Masakazu Konishi
587:Jörg-Peter Ewert
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435:
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419:
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406:Schurr, Avital,
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57:neurotransmitter
53:incubation fluid
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1432:Neurophysiology
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1369:Neurotechnology
1364:Neuroplasticity
1359:Neuromodulation
1354:Neuromanagement
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1248:Neurophilosophy
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1125:Neuropsychology
1086:
1079:
1040:Neuropsychiatry
1000:Neuroimmunology
985:Neurocardiology
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954:
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936:Neurophysiology
926:Neuromorphology
881:Neural decoding
822:
815:
797:
792:
762:
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679:Vision in toads
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631:
582:Erich von Holst
577:Karl von Frisch
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1379:Self-awareness
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1334:Neurodiversity
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1045:Neuroradiology
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1025:Neuropathology
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1015:Neuro-oncology
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901:Neurochemistry
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572:Donald Kennedy
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567:Donald Griffin
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557:Niko Tinbergen
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367:Brain Research
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300:www.google.com
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250:(5): 600–612.
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203:(3): 423–436.
197:Brain Research
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1263:Neurotheology
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1065:Neurovirology
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911:Neurogenetics
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846:Brain-reading
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841:Brain mapping
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562:Konrad Lorenz
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449:Neuroethology
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1344:Neuroimaging
1339:Neurogenesis
1223:Neurohistory
1188:Neurobiotics
1087:neuroscience
1055:Neurosurgery
980:Epileptology
962:neuroscience
931:Neurophysics
921:Neurometrics
896:Neurobiology
891:Neuroanatomy
861:Connectomics
795:Neuroscience
750:
738:
719:Lateral line
669:Waggle dance
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607:Eric Knudsen
472:
412:Kopf Carrier
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155:decapitation
147:
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85:
24:
20:
18:
1213:Neuroethics
1060:Neurotology
643:Patch clamp
612:Eric Kandel
592:Franz Huber
463:Feedforward
144:Limitations
109:respiration
105:anaesthesia
75:Mouse brain
65:antagonists
25:brain slice
1426:Categories
1374:Neurotoxin
1075:Psychiatry
617:Nobuo Suga
532:in insects
178:References
130:modulators
1319:Neurochip
1085:Cognitive
1010:Neurology
395:220923208
160:microglia
89:microtome
49:perfusion
1398:Category
1282:Concepts
1228:Neurolaw
960:Clinical
740:Category
480:Instinct
456:Concepts
414:, Vol 15
387:32755603
348:38926390
339:11208608
225:30478336
166:See also
95:Benefits
61:agonists
1410:Commons
823:science
811:History
806:Outline
752:Commons
657:Systems
636:Methods
282:2616816
274:2780225
217:1111846
134:in situ
119:of the
101:in vivo
45:circuit
1146:fields
540:People
474:Umwelt
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223:
215:
821:Basic
391:S2CID
278:S2CID
221:S2CID
27:is a
383:PMID
371:1747
344:PMID
270:PMID
213:PMID
63:and
19:The
375:doi
334:PMC
326:doi
260:hdl
252:doi
248:414
205:doi
31:in
23:or
1428::
410:,
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308:^
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201:85
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195:.
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117:pH
787:e
780:t
773:v
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434:t
427:v
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377::
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328::
302:.
284:.
262::
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227:.
207::
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